Microbiome profiles on multiple sample types from patients with non-small cell lung cancer.

This is an observational prospective study of patients undergoing curative surgery for NSCLC. Samples were collected from multiple body sites, including tumour, adjacent tissue, blood, and saliva. Extracted DNA underwent nested PCR amplification of the 16S rRNA gene and was then sequenced on the Illumina MiSeq platform. A subset of samples were quantified using QIAcuity digital PCR and further analyzed by metagenomic sequencing (MGS). Microbiome profiles were analyzed by sample type, in duplicate, and in relation to controls. All saliva samples also underwent full-length sequencing of the 16S rRNA gene, and differences in bacterial diversity and community composition with clinical features and cancer outcomes were examined.