Improved Structure and Function in Autosomal Recessive Polycystic Rat Kidneys with Renal Tubular Cell Therapy - Table 1

Cyst number was quantified in blinded images at 4 days. At 7 days, the cells were fixed in 4% paraformaldehyde, permeabilized in 0.1% Triton and incubated with rhodamine phalloidin (1:200) and Hoescht 3342 (both Life Technologies, Carlsbad, CA). Two photon images were acquired with an Olympus Fluoview FV-1000 MPE system (Olympus America, Central Valley, PA) using 839 nm excitation wavelength and an Olympus XLPLN 25x, NA 1.05 water immersion objective. Z-stacks were collected to evaluate cyst formation in 3D. Volume rendering was performed using Amira software (FEI, Burlington, MA).
Improved Structure and Function in Autosomal Recessive Polycystic Rat Kidneys with Renal Tubular Cell Therapy - Table 1