rmMCP-6 and 7 induce tube formation by endothelial cells.

SVEC4-10 cells were cultured for 5 hours at 37°C on Geltrex® in the presence of rmMCP-6 (A), rmMCP-7 (B) or in the absence of tryptases (C). (A) In the presence of rmMCP-6 most of the SVEC4-10 cells were spread on the substrate, tube formation was observed (arrowhead) and loops were also present (L). (B) When the SVEC4-10 cells were cultured with rmMCP-7, tubes (arrowheads) and loops (L) were more prevalent. (C) When SVEC4-10 cells are cultured in the absence of tryptases (control) only a few cells were seen in the initial phase of tube formation (arrowhead) and most of the cells remained unspread (arrow). Cells were stained with phalloidin conjugated to Alexa 488. Five independent experiments were performed.