Analysis of MACC1 dependent gene expression regulation in CRC cells. Homo sapiens

We employed whole genome expression profiling to identify differential gene expression in the colorectal cancer (CRC) cell line SW480 (ATCC - CCL-228), dependent on the expression level of MACC1 (Metastasis Associated in Colon Cancer 1). SW480 cells with endogenously low expression levels of MACC1 were transfected either with CMV-promoter driven MACC1-cDNA or the empty vector, and selected for stable expression. Overall design: Total RNA of two biological replicates of both MACC1 overexpressing cells and vector control cells, respectively, was isolated and subjected to whole genome expression profiling.