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A novel mNGS based-method for quantification of Microbes

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NIAID Data Ecosystem2026-05-01 收录
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This study developed a new approach, Kingcreate-Quantification (KCQ) system, for quantitative analysis of microbes in clinical specimens by mNGS, which co-sequenced DNA extracted from the specimens with synthetic dsDNA molecules used as Internal-Standard (IS), and allowed the conversion of microbial reads into their copy numbers based on the IS reads by mathematical model.

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2023-07-26
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