Comparison of pre-leukemic and leukemic H9M AML clones from murine splenocytes

The tracking of leukemic clones in acute myeloid leukemic promisses deeper insights into disease development and therapeutic options. We therefore established a fluorescent genetic barcoding (FGB) labeling approach that allows for flow cytomtric tracking of color-coded clones in vitro and in vivo. In Hoxa9 and Meis1 (H9M) dependent murine AML, we tracked the growth behavior of 24 clones in parallel and enriched for pre-leukemic clones as well as their de novo expanded counterparts and stably expanded clones from leukemic mice by fluorescence-activated cell sorting. These samples were subjected toRNA sequencing for the assessment of transcriptional changes underlying clonal maintenance and expansion. Overall design: 9 samples were analyzed. 3 pre-leukemic clones were derived from a single mouse. Pairs of 3 de novo expanded clones and stably expanded clones were enriched from 3 mice at their leukemic endpoint.