Expression data from S100A9 and HMGB1 treated human CD14+ monocytes

TLR4 signaling was reported to be involved in the upregulation of PD-L1 expression and induction of immunosuppressive properties of human CD14+ monocytes. Damage associated molecular patterns (DAMPs) S100A8, A9, and HMGB1, which act as endogenous TLR4 ligands, drive MDSC activation and were shown to be markedly expressed in the tumor microenvironment (TME) of solid tumors. The role of S100A8/9 and HMGB1 in the acquisition of MDSC immunosuppressive activity remains to be better defined. CD14+ monocytes were treated with recombinant (r) S100A9 and HMGB1 in the presence of GM-CSF with or without FPS-ZM1 or TAK-242 for 72 h. Monocytes were also stimulated with GM-CSF only for 72h (Control). After 72h, total RNA was isolated from monocytes and gene expression profiling was performed.