Functional assessment of a new PBX1 variant through CRISPR-Cas9 gene editing

To assess the role of a variant of unknown significance in PBX1 (p.(Arg107Gln) by generating a HEK293T-derived cell line knockdown for PBX1 (clone 22 cells) through CRISPR-Cas9. We then transfected the KD cells or the WT HEK293T cells with a plasmid encoding either WT or Arg107Gln PBX1 proteins. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT HEK293T cells and clone 22 cells after transient transfection with plasmid encoding WT PBX1 (WT) or Arg107Gln PBX1 (MUT)