Micropropagation of lavender: a protocol for production of plantlets

ABSTRACT Lavender (Lavandula dentata) has several medicinal applications, both in the fresh form and after industrial processing. Productivity increase could be obtained through the mass propagation, expanding the possibility of obtaining raw material for production of essential oil. The objective of this work was to develop a micropropagation protocol from explants obtained from seeds. Aspects of in vitro germination, multiplication, elongation and rooting were evaluated and, in sequence, the acclimatization process as well. The in vitro germination of lavender seeds can be obtained using a medium composed of agar and water. For multiplication, the use of MS medium (Murashige & Skoog, 1962) added with 5.0 µM 6-benzylaminopurine to the nutrient medium is the most appropriate. For elongation and rooting, the use of growth regulators is unnecessary. Acclimatization can be obtained directly by transferring the explant to plastic bags with substrate, kept in a greenhouse. It was possible to develop a protocol for the in vitro cultivation (establishment, multiplication, elongation, rooting and acclimatization) of lavender from explants of seed origin.摘要 齿叶薰衣草（Lavandula dentata）无论鲜品还是经工业加工后，均具备多种药用用途。通过规模化繁殖可提升其产量，为精油生产拓展原料获取渠道。本研究旨在建立一套以种子来源外植体为材料的薰衣草微繁规程。研究对离体萌发、增殖、伸长与生根环节进行了系统评价，并同步开展了驯化流程的优化。结果显示，薰衣草种子的离体萌发可采用仅含琼脂与水的培养基；增殖阶段，向培养基中添加5.0 µM 6-苄氨基腺嘌呤的MS培养基（Murashige & Skoog，1962）为最优选择；伸长与生根阶段则无需添加生长调节剂；驯化环节可直接将外植体移栽至装有栽培基质的塑料袋中，置于温室环境培育。本研究成功建立了一套涵盖外植体建立、增殖、伸长、生根及驯化全流程的薰衣草离体培养规程，适用于种子来源的外植体。