Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.40A resolution

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site fMEAAAKC-peptidyl-tRNAcys at 2.40A resolution Descriptor: 16S Ribosomal RNA, 23S Ribosomal RNA, 30S ribosomal protein S10, ... Authors: Aleksandrova, E.V, Syroegin, E.A, Basu, R.S, Vassilevski, A.A, Gagnon, M.G, Polikanov, Y.S. Deposit date: 2025-01-12 Release date: 2025-06-18 Last modified: 2026-08-12 Method: X-RAY DIFFRACTION (2.4 Å) Cite: Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome. Science, 388, 2025野生型嗜热栖热菌（Thermus thermophilus）70S核糖体与mRNA、A位点Q230-N5甲基化释放因子1以及P位点fMEAAAKC-肽酰-tRNAcys结合的晶体结构，分辨率达2.40埃 描述符：16S核糖体RNA（16S Ribosomal RNA）、23S核糖体RNA（23S Ribosomal RNA）、30S核糖体蛋白S10（30S ribosomal protein S10）…… 作者：Aleksandrova, E.V.、Syroegin, E.A.、Basu, R.S.、Vassilevski, A.A.、Gagnon, M.G.、Polikanov, Y.S. 提交日期：2025-01-12 发布日期：2025-06-18 最后修改日期：2025-07-02 实验方法：X射线衍射（X-RAY DIFFRACTION）（2.4 Å） 引用文献：《核糖体上释放因子介导的肽酰-tRNA水解机制》（Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome），《科学》（Science），第388卷，2025年