Data for: Evidence to support phytosanitary policies - The minimum effective heat treatment parameters for pathogens associated with forest products
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Research on reducing the movement of pests on wood products has led to several options for safer trade including heat treatment of wood to mitigate pests. In this study, pathogenic organisms commonly regulated in the trade of forest products were tested to determine the minimum heat dose (temperature and time) required to cause mortality. The mycelial stage of tree pathogens, Heterobasidion occidentale, Grosmannia clavigera, Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum and P. xmultiformis, which may be found in wood products, were tested in vitro using the Humble water bath with parameters simulating the rate of heat applied to wood in a commercial kiln. The lethal temperature for the pathogens ranged from 44 to 50°C for a 30-minute treatment duration. A molecular diagnostic method to confirm pathogen mortality was available for five of the species heat treated. RNA detection using reverse transcription real-time PCR was used to validate pathogen mortality fol..., The data are observations on post-treatment survival of Heterobasidion occidentale, Grosmannia clavigera, Bretziella fagacearum, Phytophthora cinnamomi, P. lateralis, P. ramorum and P. xmultiformis exposed to heat in a water bath. The experiments were used to determine the optimal heating conditions for treatment of wood infested with forest pathogens. The mycelial stage of pathogens growing on grain were exposed to temperatures from 40 up to 52 degrees celsius for 30 minutes. Heat-treated and unexposed control grains with mycelium were plated on 20% V8 agar for Phytophthora species or 2% malt extract agar with yeast extract for fungi and observed for growth to determine if they survived treatment at each of the exposure temperatures., , # Data for: Evidence to support phytosanitary policies -The minimum effective heat treatment parameters for pathogens associated with forest products [https://doi.org/10.5061/dryad.7m0cfxq3k](https://doi.org/10.5061/dryad.7m0cfxq3k) Data are observations on post-treatment survival of *Heterobasidion occidentale*, *Grosmannia clavigera*, *Bretziella fagacearum*, *Phytophthora cinnamomi*, *P. lateralis*, *P. ramorum* and *P. xmultiformis* exposed to heat in a water bath at temperatures ranging from 40 up to 52 degrees Celsius for 30 minutes. # Description of the data and file structure These data were assembled and summarized from raw data sheets and transferred into excel format. Description of fields: * Experiment number: The experimental run within which the data were collected * Experiment date: The date on which the experimental run was conducted * Species: The Latin name of the pathogen containing the first initial of the genus followed by the species name * Isolate...
针对降低木制品上有害生物迁移的研究已催生多种更安全的贸易方案，其中包括通过木材热处理以抑制有害生物传播。本研究针对林产品贸易中通常受管制的病原生物开展测试，旨在确定导致其死亡所需的最低热处理剂量（温度与作用时长）。 本实验针对木制品中可能检出的林木病原菌菌丝体阶段，包括西方异担子菌（*Heterobasidion occidentale*）、棒形帚枝霉（*Grosmannia clavigera*）、栎树猝死病原菌（*Bretziella fagacearum*）、肉桂疫霉菌（*Phytophthora cinnamomi*）、P. lateralis、P. ramorum 以及 P. xmultiformis，采用恒温水浴（Humble water bath）进行体外测试，模拟商用窑炉中木材受热的速率参数。经30分钟处理后，该类病原菌的致死温度范围为44至50℃。针对其中5个经热处理的物种，可采用分子诊断方法验证其死亡情况。研究采用反转录实时PCR（reverse transcription real-time PCR）进行RNA检测以验证病原菌死亡率[原文后续内容存在截断]。 本数据集记录了经水浴热处理后的上述7种病原菌的存活情况观测数据，实验旨在确定侵染林木病原菌的木材最优热处理条件。将谷物培养基上生长的病原菌菌丝体置于40至52℃的温度下处理30分钟，随后将经热处理与未暴露的带菌丝体对照谷物分别接种于疫霉菌专用的20%V8琼脂培养基，或真菌专用的含酵母提取物的2%麦芽浸膏琼脂培养基，通过观察菌落生长情况以判断各暴露温度下病原菌是否存活。 # 数据集关联：支撑植物检疫政策的证据——林产品相关病原菌的最低有效热处理参数 [https://doi.org/10.5061/dryad.7m0cfxq3k](https://doi.org/10.5061/dryad.7m0cfxq3k) 本数据集记录了经恒温水浴热处理的*Heterobasidion occidentale*、*Grosmannia clavigera*、*Bretziella fagacearum*、*Phytophthora cinnamomi*、P. lateralis、P. ramorum 及 P. xmultiformis的存活观测数据，处理温度范围为40至52℃，处理时长为30分钟。 # 数据说明与文件结构 本数据集从原始实验记录表整理汇总，并转换为Excel格式。 字段说明： * 实验编号：采集数据所属的实验批次 * 实验日期：实验批次开展的日期 * 物种：病原菌的拉丁学名，格式为属名首字母缩写后接种名 * Isolate...
