Zea mays Transcriptome or Gene expression. Zea mays

Maize seedling transcriptome responses to six abiotic perturbations (heat, cold, darkness, desiccation, salt, UV-B) and 2 controls were analyzed Keywords: comparitive transcriptome profiling Overall design: Tissues were grown at Stanford University during the summer of 2005. RNA was extracted and purified, cDNA generated, and amplified cRNA was labeled with Cy3 or Cy5 dyes from 4 biological replicates of each treatment. Each replicate was composed of a pool of 10 similarly treated whole seedlings (with the endosperm and kernel removed). Two types of biological controls were employed: 1) seedlings germinated under constant 26ºC temperature, with 16 h light and adequate water and 2) field grown juvenile leaf samples Field seedlings emerged on day 6 or 7.