Strand-Specific RNA Sequencing for wild type and Drpd3 yeast entering quiescence [RNA-seq]

Strand-specific RNA sequencing was performed on wild type yeast in log phase, after diauxic shift, and after entry into quiescence with incorporation of external ERCC RNA spike-in controls to account for global changes in RNA abundance本研究针对野生型酵母的三个生长阶段——对数生长期、二次生长转变后以及进入静止期——开展了链特异性RNA测序（Strand-specific RNA sequencing），实验中掺入了外部ERCC RNA掺入对照（external ERCC RNA spike-in controls），以校正RNA丰度的全局变化。