RNA-seq experiment performed on activated human CD4+ T cells

Analysis perfomed on four paired samples with two treatments and paired untreated sample We purified naïve CD45RA+CD4+ T cells from PBMCs collected from SLE patients. These cells were maintained for two days in culture medium supplementd with 20 IU of IL-2. Thereafter cells were harvetd and total RNA was purified using the kit from InVitroen. The sequencing was perfomed using Ion Torrent Kit. The RNA-seq was perfomed on Ion Torrent Sequncer.