Gene expression profile of macrophages in aneurysm lesions at the growth phase.

Recent experimental studies have defined intracranial aneurysms as a macrophage-mediated chronic inflammatory disease affecting intracranial arteries. We used single cell RNA sequencing (scRNA-seq) to analyze the population of macrophages in aneurysm lesions at the growth phase. The previously-established aneurysm model of rats was used. Macrophages were labeled with the fluorescent protein and isolated by a laser-microdissection method and analyzed using scRNAseq.