\"Genome-wide microsatellite marker development from next-generation sequencing of two non-model bat species impacted by wind turbine mortality: Lasiurus borealis and L. cinereus (Vespertilionidae)\" in Genomic Resources Notes accepted 1 October 2013 to 30 November 2013

Tree-roosting bats in the genus Lasiurus are widespread, migratory species that have not been well characterized for population genetic diversity and structure due to a lack of genetic resources. Generating genetic resources in Lasiurus is made pressing by the need for conservation genetic assessments of demographic trends in this genus, which comprise a large percentage of bat mortalities at wind turbine sites across North America. We report on marker development from whole-genome Illumina sequencing of the red bat (Lasirus borealis) and the hoary bat (L. cinereus). We generated paired-end libraries for a single individual of each species, sequenced on the Illumina HiSeq platform. We mapped a total of 46.6 million reads to the Myotis lucifigus reference genome, and used bioinformatics searches to identify tends of thousands of simple sequence repeats (SSRs) distributed across the bat genome. We selected 48 candidate microsatellite loci to develop cross-species primer sequences for Lasi...棕蝠属（Lasiurus）的树栖蝙蝠为分布广泛的迁徙类群，由于遗传资源匮乏，其种群遗传多样性与群体结构尚未得到充分解析。鉴于该类群在北美风力涡轮机作业区域的蝙蝠死亡事件中占比极高，开展针对其种群动态趋势的保护遗传学评估的需求，使得构建棕蝠属遗传资源的工作迫在眉睫。本研究通过对红蝠（Lasiurus borealis）与灰红蝠（L. cinereus）开展全基因组Illumina测序开发分子标记。我们为每个物种的单个个体构建了双端测序文库，并通过Illumina HiSeq平台完成测序。我们将总计4660万条测序reads比对至小鼠耳蝠（Myotis lucifugus）参考基因组，并通过生物信息学分析检索到分布于蝙蝠全基因组的数以万计的简单序列重复（SSRs）位点。我们筛选出48个候选微卫星位点，以开发棕蝠属的跨物种引物序列，用于……