RNA-seq of Pluripotent stem cell derived cardiac cells

Cardiac fibroblasts (CFBs) support heart function by secreting extracellular matrix and paracrine factors, respond to stress associated with injury and disease, and therefore are an increasingly important therapeutic target to treat heart disease. Here, we profile how developmental lineage of human pluripotent stem cell-derived CFBs, epicardial (EpiC-FB) and second heart field (SHF-FB) impacts their transcriptome. We compared this to human pluripotent stem cell derived cardiomyocytes, primary fetal cardiac fibroblasts, primary adult left ventricular fibroblasts, and primary adult dermal fibroblasts. We used three independent differentiation replicates in H9 hESCs for the hPSC-derived cell types and hPSC-cardiac fibroblasts were sampled at P1. Three technical replicates of primary fibroblasts were used between P3-5.