Predicted Cdk2 substrates in the cyclin E1-interactome.

The fraction of predicted Cdk substrates among cyclin E1-interactors identified in this study, versus in the entire proteome present in mouse SwissProt database. Cdk substrates were predicted using Scansite 3.0 using either high-stringency scoring (high-confidence; median score = 0.252) or medium-stringency scoring (med-confidence; median score = 0.341). Median score in the search of the entire proteome was 0.536, indicating even less stringent criterion than the above medium-stringency search. The fractions were compared using one-tailed Fisher’s exact test.