The Missing Link: Connecting Cultivation Conditions and Refolding Performance via Inclusion Body Biophysical Properties

This dataset accompanies the article titled "The missing link: Connecting cultivation conditions and refolding performance via inclusion body biophysical properties" accepted by Biotechnology and Bioengineering. The study investigates the impact of cultivation conditions on the formation and properties of inclusion bodies (IBs) in Escherichia coli, and their subsequent refolding performance. The dataset includes raw and processed data from 24 cultivations of a proprietary industrial E. coli Lonza XS® strain expressing an anti-desipramine single-chain variable fragment (scFv) antibody. The cultivations were performed using a Design of Experiments (DoE) approach, varying post-induction temperature, pH, and feed rate. Key data included in this dataset: Cultivation Data: Measurements of titer, purity, cell dry weight (CDW), and other cultivation performance indicators. Biophysical Characterization: Data from fluorescence spectroscopy, FT-IR spectroscopy, Raman spectroscopy, and laser diffraction (LD) measurements of the IBs. Analytical Measurements: Capillary gel electrophoresis (CGE)-SDS and size exclusion chromatography (SEC) HPLC data for protein quantification and characterization. Refolding Data: Yields of the refolding process under different conditions. Statistical Analysis: Scripts for statistical modeling and analysis of the whole dataset. This dataset provides a comprehensive resource for researchers interested in the biophysical properties of inclusion bodies, the impact of cultivation conditions on protein production, and the optimization of refolding processes.