Supplemental material for Non-pasteurized milk enhances early colonization and persistence of Lactobacillus species in dairy calves promoting a stable hindgut microbiome

Figure S1. Fecal microbial composition of the CON and HT groups over time. (a-b) Stacked bar plots of species distribution at phylum and genus levels. (c) Absolute abundance of L. johsonii (U.test, P<0.01). Figure S2. Effect of pasteurization on the microbiota of milk. (a-b) Composition of colostrum and raw milk microorganisms at the phylum level. (c-d) Determination of viable bacteria in colostrum and milk before and after pasteurization. Figure S3. Total bacterial isolates and growth phenotypes of L. reuteri (N=40). (a-c) The growth capacity of L. reuteri on glucose. (d-f) The acid production capacity of L. reuteri. (a) The strains were from the feces of CON group calves. (b) The strains were from the feces of HT group calves. (c) The strains were from the unpasteurized milk. Table S1. Bacterial primers used to estimate the density of bacteria in the calf feces. A detailed description of each of the 5Qs.补充图S1. 对照组（CON）与高处理组（HT）粪便微生物群随时间的组成变化。(a-b) 门水平与属水平物种分布的堆叠柱状图。(c) 约氏乳杆菌（L. johsonii）的绝对丰度（U检验，P<0.01）。 补充图S2. 巴氏灭菌对牛乳微生物群的影响。(a-b) 初乳与生牛乳微生物群的门水平组成。(c-d) 巴氏灭菌前后初乳与牛乳中的活菌数测定结果。 补充图S3. 罗伊氏乳杆菌（L. reuteri）的总分离菌株与生长表型（N=40）。(a-c) 罗伊氏乳杆菌在葡萄糖培养基上的生长能力。(d-f) 罗伊氏乳杆菌的产酸能力。(a) 所用菌株分离自对照组犊牛粪便。(b) 所用菌株分离自高处理组犊牛粪便。(c) 所用菌株分离自未灭菌牛乳。 补充表S1. 用于定量犊牛粪便中细菌密度的细菌引物。 5个问题项的详细说明。