Diet-induced obesity dysregulates rapid epigenetic response to hypoxia in macrophages (ChIP-Seq)

Macrophages play important roles in tissue homeostasis and tissue healing process. The response to hypoxic environment is a trigger to induce key signaling pathways in macrophages. Functions of macrophages are dysregulated in metabolic diseases such as diabetes and obesity resulting in poor tissue healing. Epigenetic status including histone modification in macrophages is regulated by hypoxia. However, whether and how obesity influences histone modification under hypoxia is unknown. Using chromatin immunoprecipitation-sequencing (ChIP-seq), RNA-seq on mouse bone marrow-derived macrophages, we sought to determine whether hypoxia alters histone 3-lysine 4 trimethylation (H3K4me3) and gene expression profile in macrophages and how obesity influences H3K4me3 enrichment on the genome in hypoxia-dependent and -independent manner. Comparative H3K4me3 marked peak analysis of ChIP-seq data for bone marrow-derived macrophages (BMDMs) obtained from C57B6/J mice fed a high-fat diet or a normal-fat diet with or without 1% hypoxia treatment for 1 hour.