OsCPK21 regulates rice pollen development

To identify the molecular mechanism of OsCPK21 regulating pollen development, a genome-wide analysis of the gene expression profiles in the rice spikelet of ZH11 and OsCPK21-RNAi transgenic plants during anther development was performed. Hybridization with the microarray and subsequent analysis showed that a total of 5020 genes displayed altered expression (at least 2-fold) under suppressed OsCPK21 expression. Among the altered genes, 1419 were up-regulated, and 3601 were down-regulated. The ZH11 and OsCPK21-RNAi (3 lines) plants were growth in the same condition. Then rice spikelets at stage 9 and 12 of anther development were chose and collected, and total RNA was extracted and amplified from these four samples.