Inflammatory gene expression profiling in peripheral blood from patients with Alzheimer’s disease reveals key pathways and hub genes with potential diagnostic utility

Late-onset Alzheimer disease (LOAD) is the most common neurodegenerative disorder worldwide. Recent studies have shown that differential expression in genes (DEG) related to inflammatory response may result associated with disease onset and progression. This study aimed to explore the molecular pathogenesis of LOAD-related inflammation through next-generation sequencing, to assess RNA expression profiles in Alzheimer's disease patients and healthy controls. RNA was isolated from whole blood from 5 LOAD cases and 10 controls (all female. Mean age 76.3±3.5). The amplicon sequencing approach using 20812 probes sets specific to human mRNA was applied to assess gene expression profiles, and functional annotation was performed.