Identification of r<i>Ts</i>-Pt by SDS-PAGE and western blotting.
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(A) SDS-PAGE analysis of the expression and purification of rTs-Pt. The gel was stained with Coomassie Brilliant blue. Lane 1, prestained protein marker (Genview); lane 2, E. coli lysate (pET28a) without IPTG; lane 3, E. coli lysate (pET28a) with IPTG; lane 4, E. coli lysate (pET-28a/Ts-Pt) without IPTG; lane 5, E. coli lysate (pET-28a/Ts-Pt) with IPTG; lane 6, supernatant of E. coli lysate (pET-28a/Ts-Pt) with IPTG; lane 7, rTs-Pt purified by Ni-affinity chromatography. (B) Western blot analysis of the antigenicity of rTs-Pt. Lane 1, rabbit anti-rTs-Pt serum; lane 2, negative rabbit serum; lane 3, T. spiralis-infected mice serum; lane 4, negative mice serum. (C) Western blot analysis of the crude somatic extracts and ES products of T. spiralis were recognised by rabbit anti-rTs-Pt serum. Lane 1, Ad crude somatic extracts; lane 2, Ad ES products; lane 3, NBL crude somatic extracts; lane 4, NBL ES products; lane 5, ML crude somatic extracts; lane 6, ML ES products.
