Summary of Trx redox status following TR1 knockdown and Sec-deficient TR1 expression: Percent Trx in each redox state (% total).

Summary of the 6 possible redox states, from the most oxidized (state 1) to the least (state 6) of Trx in RKO cells with endogenous TR1 (si-Scramble), with endogenous TR1 knocked-down (si-TR1), or endogenous TR1 knocked-down but with induced Sec-deficient mutated TR1 (si-TR1+mTR1). Since the majority of the Trx was found to be in the reduced state, we stimulated with 1 mM diamide for 30 min to oxidize the cells and those cells without endogenous TR1 display more oxidized Trx than cells with endogenous TR1.