Transcriptome profile of rice root in response to external ammonium supply

Sterilized NIP seeds were germinated in water under long day condition (14h photoperiod, 70% humidity, 28C) for 3 days, and then transferred to hydroponic cultures supplied with modified Kimura B solution containing 0.125 mM or 1.25 mM (NH4)2SO4 for 6h and 24h. At each time point, the root tip segments (~1 cm), comprising root meristem zone and elongation zone, from 40 NIP seedlings were excised and collected as one biological repeat. Three independent biological repeats were used for transcriptome analysis.