Segmented anisotropic ssTEM dataset of neural tissue

We provide two image stacks where each contains 20 sections from serial section Transmission Electron Microscopy (ssTEM) of the Drosophila melanogaster third instar larva ventral nerve cord. Both stacks measure approx. 4.7 x 4.7 x 1 microns with a resolution of 4.6 x 4.6 nm/pixel and section thickness of 45-50 nm.
In addition to the raw image data, we provide for the first stack a dense labeling of neuron membranes (including orientation and junction), mitochondria, synapses and glia/extracellular space. The first stack serves as a training dataset, and a second stack of the same dimension can be used as a test dataset.
All the data and further information is available in the GitHub repository linked below.

本数据集提供两组图像栈，每组包含来自黑腹果蝇三龄幼虫腹神经索的连续切片透射电子显微镜（ssTEM）的20个切片。两组图像栈的尺寸约为4.7 x 4.7 x 1微米，具有4.6 x 4.6纳米/像素的分辨率和45-50纳米的切片厚度。除原始图像数据外，对于第一组图像栈，我们还提供了神经元膜（包括方向和连接点）、线粒体、突触和胶质细胞/细胞外空间的密集标记。第一组图像栈作为训练数据集，尺寸相同的第二组图像栈则可用作测试数据集。所有数据及相关信息均可在以下GitHub仓库中获取。