In vivo transciptomic responses to bromobenzene exposure from liver and kidneys of Sprague Dawley rats

In this study we tested the ability to identify early perturbations in the organ (liver and kidney) metabolism due to bromobenzene exposure at two early time points (5 and 10 h) using RNA-sequencing. Overall design: We treated Sprague Dawley rats with a single dose of bromobenzene (0.4 g/kg) with corresponding controls given 2% saline as vehicle (n =8 each for control and treatment). Liver and kidney samples for RNA-sequencing were collected 5 and 10 h post bromobenzene/vehicle treatment. Total 32 rats were used with 8 replicates for each condition and time point.