GOM open ocean (GOMOO): Illumina MiSeq sequenced 16S library of Microbial Community Response to Oil and Corexit

Mesocosm experiments were carried out to test the effect of oil and Corexit on microbial community composition and structure over time. Four mesocosm treatments were prepared in triplicate for this study: (1) Control (seawater only); (2) WAF (oil + seawater); (3) Chemically-enhanced WAF (CE-WAF; oil + Corexit + seawater); (4) Diluted CE-WAF (1:10 dilution). Starting at time-point zero and every 12 hours thereafter, water was collected from each mesocosm and filtered (47mm, 0.2um PES membranes). DNA was extracted from filtered water samples and sequenced using barcoded primers specific for the V4 hypervariable region of the 16S rRNA gene.本研究开展中宇宙实验（mesocosm），旨在探究石油与科息特（Corexit）消油剂随时间推移对微生物群落组成与结构的影响。本研究共设置4组中宇宙处理，每组设置3个生物学重复：(1) 对照组（仅海水）；(2) 水包油组分组（WAF，即石油+海水）；(3) 化学强化型水包油组分组（CE-WAF，即石油+科息特消油剂+海水）；(4) 稀释型化学强化水包油组分组（按1:10比例稀释的CE-WAF）。自时间零点起，后续每隔12小时从每个中宇宙系统采集水样，使用直径47mm、孔径0.2μm的聚醚砜（PES）膜进行过滤。随后从过滤后的水样中提取基因组DNA，采用靶向16S核糖体RNA（16S rRNA）基因V4高变区的带条形码引物完成扩增测序。