five

Treatment groups and schedule of the cage experiments.

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The N. ceranae inoculum contained 100,000 N. ceranae spores in 50% (w/v) sucrose solution. The control bees received the N. ceranae-free suspension as well in 50% (w/v) sucrose solution. The pupae extract from BQCV-free pupae was administered in 50% (w/v) sucrose solution and BQCV inoculum was composed of highly concentrated BQCV suspension of infected pupae in 50% (w/v) sucrose solution. Each bee was individually inoculated with 5 µl of the respective treatment suspensions (reps.  =  replicates).
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2014-01-17
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