data and script for Banousse et al: <b>Genetic and environmental basis of transcriptional thermal plasticity of brook charr fry</b>

<b>DB_Brain_chip_Laval_calculation.xlsx</b>: This file contains the calculation of N0 (following Tuomi et al. 2010) and Dct (DN₀) derived from the Crt, which is the fractional PCR cycle number where fluorescence crosses the quantification threshold.<b>DB_dct_Brain_chip_Laval_Box_Cox.csv</b>: This file includes the dct transformed values (using Box-Cox transformation) for each of the 10 genes (AVT1, BDNF, CART, CCK.L, CIRBPA, DCX, NEUROD1, NPY, PCNA, SOX2).<b>Code_LMM_Brain_chip_Laval.Rmd</b>: This script contains the code used to assess the Linear Mixed Models (LMM) for each of the 10 genes analyzed.<b>data_for_ASREML.zip:</b> This zip file contains the same data as <b>DB_dct_Brain_chip_Laval_Box_Cox.csv</b>, but it is divided based on the parental thermal regimes (cold and warm). Additionally, it includes the pedigree matrix. These files are used in the (<b>ASREML_Brain_chip_Laval.Rmd)</b> to perform quantitative genetic analysis and partition the variance in gene expression into genetic, environmental, and parental components.<b> </b><b>ASREML_Brain_chip_Laval.Rmd: the code script used for the quantitative genetic analysis</b><b>data_for_G X E _analysis.zip : </b>This zip file contains the data used in the <b>GxE_Analysis_Brain_chip_Laval.Rmd</b> script to assess the genotype-by-environment analysis. It includes data exclusively for full-sib families, treated as a single genotype.<b>G X E Analysis_Brain_chip_Lval.Rmd</b><b>: </b>This script contains the code used to perform the genotype-by-environment (GxE) analysis.<br>