Single cell RNA sequencing of the lung pre-metastatic niche in a syngeneic mouse model of rhabdomyosarcoma

C57BL/6 mice were injected orthotopically with M3-9-M ffluc-mCherry rhabdomyosarcoma tumor cells in the gastrocnemius muscle of the leg. Mice were treated with 8E6 IL12-secreting genetically engineered myeloid cells (IL12-GEMys) on day 12 post tumor inoculation. Lungs were harvested 3 days post IL12-GEMy treatment, 15 days post primary tumor inoculation. 3' single cell RNA-Seq was performed on isolated cells. Overall design: Single cell 3' RNA-Seq from lung tissue of naive mice, primary tumor-bearing pre-metastatic mice, and primary tumor-bearing pre-metastatic mice treated with IL12-GEMys.