Additional file 1 of Neuromolecular responses in disrupted mutualistic cleaning interactions under future environmental conditions

Additional file 1: Table S1. Seawater physicochemical parameters in the experimental setups for (N=24) L. dimidiatus and (N=24)A. leucosternon individuals exposed to four conditions: one i) control with present day environmental conditions (29 °C, pH 8.1, pCO2 ~ 400 μatm), ii) warming scenario referred to as elevated ‘warming (32 °C, pH 8.1, pCO2 ~400 μatm), iii) ocean acidification referred to as ‘high CO2’ (29 °C, pH 7.7, pCO2 ~1000 μatm) and a combined condition of iv)‘warming & high CO2’ (32 °C, pH 7.7, pCO2 ~1000 μatm). These parameters follow the IPCC’s RCP scenario 8.5. Table S2. Individual brain dissections data for L. dimidiatus and A. leucosternon for each environmental condition. Brain regions dissected: forebrain (FB), midbrain (MB) and hindbrain (HB). Standard length (SL) and Weight (W) is provided for each individual used in the study. Table S3a. Behavioural trial data for each aquarium setup for individuals of L. dimidiatus and A. leucosternon assigned for control, warming, high CO2 and warming & high CO2. NCBI Biosample accession numbers of behavioural trials are provided. Table S3b. Analysis of Deviance Table (Type II tests) for each behavioural attribute tested during behavioural trials between L. dimidiatus and A. leucosternon. Significant values (*) considered with a p<0.05.Table S3c. Contrast of the post hoc multiple comparisons based on environmental conditions tested: Control, high CO2, warming and warming & high CO2. Significant values (*) considered with a p<0.05. Table S4. Enriched Gene Ontology (GO) terms for the differentially expressed genes shared among conditions of warming, high CO2 and warming & high CO2 for L. dimidiatus (N=672). Table S5. Enriched Gene Ontology (GO) terms for the differentially expressed genes shared among conditions of warming, high CO2 and warming & high CO2 for A. leucosternon (N=30). Table S6a. Specific Enriched Gene Ontology (GO) terms expressed for L. dimidiatus during the warming condition. Table S6b. Enriched Gene Ontology (GO) terms expressed the Forebrain region (FB) of L. dimidiatus during the warming condition. Table S6c. Enriched Gene Ontology (GO) terms for the Midbrain region (MB) of L. dimidiatus during the warming condition. Table S7. Specific Enriched Gene Ontology (GO) terms expressed for A. leucosternon during the warming condition. Table S8. Specific Enriched Gene Ontology (GO) terms expressed for L. dimidiatus during the high CO2 condition. Table S9. Specific Enriched Gene Ontology (GO) terms expressed for A. leucosternon during the high CO2 condition. Table S10a. Differential expressed genes found for L. dimidiatus during the warming &high CO2 condition (4581). Table S10b. Specific Enriched Gene Ontology (GO) terms expressed for L. dimidiatus during the warming & high CO2 condition. Table S11. Specific Enriched Gene Ontology (GO) terms expressed for A. leucosternon during the warming & high CO2 condition.