A combination of organic acids and glucose prime a late stage of fungal biotrophy

We show that combinations of organic acids and glucose trigger phenotypes associated with the late stage of biotrophy for the maize pathogen Ustilago maydis. These phenotypes include the expression of a set of effectors normally observed only during biotrophic development as well as the formation of melanin associated with sporulation in plant tumors. Ustilago Maydis 521 grown in a combination of carbon sources (glucose or glocose + malate) with and without aeration sampled at 24 and 72 hours. 3 replicates are sequenced for each group.