Transcriptional Idenetity of Itch-activated Central Amygdala Neurons

We performed RNA-seq to identify transcriptional profiles of itch-activated CeA neurons. To do this, we crossed FosTRAP mice to the Cre-dependent tdTomato flox-stop reporter line as described above. We then injected chloroquine into the nape of the neck, paired with injection of 4-OHT to FosTRAP expression of tdTomato in itch-activated neurons. The CeA was harvested, the cells dissociated and sorted by FACS, and tdTomato+ cells were compared to TdTomato- cells. Facs CeA TdTomato+ve and tdTomato _ve neurons after TRAPping these neurons to chloroquine stimuli.