The comparison of positively selected sites derived from 454^a and Sanger clone-based sequences.

Forty-six amino acid consensus sequences (subtype A1) from 454 and Sanger clone-based methods are subjected to positive selection analysis by random effect likelihood (REL) method. The result of FEL analysis is given as Bayes factor and possibility of positive selection. The differences of positively selected sites identified between two amplified sequence populations are shown in bold. a: 454 pyrosequencing; b: Bayes factor value (>100) is deemed as positive selection; c: positive selection.