Illumina reads analysis of Vibrio alfacsensis pSEA2-free strain LN95

Illumina reads of Vibrio alfacsensis pSEA2-free strain LN95 were used to confirm the loss of plasmid pSEA2 from strain 04Ya249. These reads were also used in search for the circular form of Tn6945, but it was not detected.

bam and bai files were posted in figshare to view mapping results in Tablet or IGV.

# result of mapping to 04Ya249_submission.fas
LN95_04Ya249_mapped.sort.bam
LN95_04Ya249_mapped.sort.bam.bai

# result of mapping to "att_seqs_04Ya249.fas"
LN95_atts_mapped.sort.bam
LN95_atts_mapped.sort.bam.bai
terminal commands, input files used for mapping and coverage analysis results were also posted. Illumina fastq files are available under SRA/DRA accession number DRA011762

.

The following two files are Sanger sequencing trace files of PCR-amplifed attTn from strain 04Ya249 genomic DNA.
attTn_type1_04Ta249-LN127-128-10_H10_016.ab1
attTn_type2_04Ta249-LN127-128-9_G10_014.ab1