Expression data from BMDC

Dendritic cells increase expression of pro-inflammatory cytokines and chemokines and undergo metabolic reprogramming upon TLR activation. We treated mouse BMDC with TLR7/8 ligand imiquimod (IMQ) and palmitic acid (PA) to reveal inflammatory and metabolic pathways that are sensitive to TLR7/8 activation and a high fatty acid environment. We used microarray to identify pathways in dendritic cells that link inflammation and metabolism in a high fatty acid environment. Bone marrow-derived dendritic cells differentiated in the presence of GM-CSF (GM-DC) were used for RNA extraction and hybridization on Affymetrix microarrays. GM-DC were treated with mock or 3 uM IMQ during 24 hr with or without 0.5 mM PA conjugated with BSA.