Video 7. Extrusion of a Mm aggregate out of the tail fin.

Double transgenic Tg(mpeg1:EGFP) and Tg(lyz:DsRed2) larvae infected with Mm were imaged alive every ~60 sec from 1 hpi to 17 hpi by CLSM (Nikon A1). The GFP-positive (green) macrophages containing a Mm (red) aggregate undergoes cell death showing fragmentation. The bacterial content is clearly extruded from the tail fin 3 hours after the macrophage showing fragmentation (Fig. 3D). The corresponding transmission channel is shown below, showing a protuberance on the outer epithelial layer, which is eventually shed off. The maximum intensity projection from 11 CLSM images (step size in z-direction, 5.56 µm) is shown at 10 frames per second. Scale bar: 20 µm.