Transcriptomic analysis provide new insight into molecular mechanisms of adventitious rooting recalcitrance in yam vine cutting

Vine cutting propagation in yams offers a transformative approach to conventional tuber-dependent cultivation, with enhanced tuber yield and quality. Adventitious root (AR) formation is a critical prerequisite for vine cutting survival, with substantial variability among yam varieties. However, relatively little is known about the regulatory mechanisms that restrict the application of cutting propagation in recalcitrant varieties. In this study, we integrated rooting rate comparisons, anatomical observations, phytohormone content determination, and transcriptomic profiling to elucidate the developmental mechanisms influencing AR formation.