Dataset for the paper entitled "A genetic map of the response to DNA damage in human cells" by Olivieri M et al. Cell 2020

We used pooled CRISPR/Cas9 screens in the human RPE1-hTERT p53-/- cell line against 27 genotoxic agents. The Dataset herein make the primary data available. The files are: * Additional genes of interest (discussion and data relating to TMEM2, ESD, USP37, PHF12, BTAF1 and DRAP1) * Supplementary Raw Data: Data used to make all the graphs in the manuscript * Folder "CRISPR screen readcount files". Readcounts for the screens undertaken as part of this study. * Folder "Raw images for immunoblots": uncropped images for all the blots in the manuscript * Folder: "Additional QC analyses of CRISPR screens": Additional analyses that measure the quality of the screens using Presion-Recall curves of essential genes and an estimate of genes that scored as hits due to batch effects. * Folder "Data_Analysis_Code" R markdown file along with data files * File: "Additional Genes of Interests" contains a short discussion of genes we found noteworthy after analysis of our screen data. Version 2 note: this version differs from Version 1 by a single file (FDRPos_31screens.csv) which has a tab error fixed.本研究以人源RPE1-hTERT p53-/-细胞系为模型，开展了覆盖27种遗传毒性试剂的混合CRISPR/Cas9筛选实验。本数据集公开全部原始实验数据，所含文件及文件夹如下： * 额外关注基因（Additional genes of interest）：包含TMEM2、ESD、USP37、PHF12、BTAF1及DRAP1相关的讨论与配套实验数据 * 补充原始数据：用于制作论文中全部图表的原始实验数据 * 文件夹"CRISPR筛选读长计数文件"：包含本研究中所有筛选实验的读长计数数据 * 文件夹"免疫印迹原始图像"：包含论文中所有免疫印迹条带的未裁切原始图像 * 文件夹"CRISPR筛选额外质控分析"：通过必需基因的精确率-召回率（Precision-Recall）曲线，以及对因批次效应被判定为阳性命中基因的评估值，对筛选实验质量进行评估的额外分析内容 * 文件夹"数据分析代码"：包含R Markdown文档及其配套数据文件 * 文件"额外关注基因（Additional Genes of Interests）"：包含对本研究筛选数据分析中发现的值得关注的基因的简短讨论 版本2说明：本版本与版本1的差异仅为修正了FDRPos_31screens.csv文件中的制表符错误。