Identification of SRSF6 splicing regulatory map and its impact on diabetes susceptibility genes regulation

The downregulation of diabetes susceptibility gene GLIS3 contributes to pancreatic beta cell demise, at least in part, through downregulation of the splicing factor SRSF6. Here, we used individual-nucleotide UV crosslinking and immunoprecipitation (iCLIP) to map the RNA binding landscape of SRSF6 in pancreatic beta cells. Overall design: Four independent iCLIP replicates for endogeneous SRSF6 from human pancreatic beta cells (EndoC-ßH1 cell line)