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Transcriptomic analysis of PerSort sorted Mycobacterium tuberculosis persister cells

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NIAID Data Ecosystem2026-03-12 收录
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https://discovery.biothings.io/dataset/9d4642688294f97e
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Dim and lit cells (n= 300,000) were PerSorted into 1 mL TriZol having ~ 1000 bone marrow derived macrophage cells. RNA was extracted with phenol chloroform method described previously (PMID:30833303), rRNA was depleted with Illumina rRNA depletion kit, and mycobacterium RNA was enriched with Path-seq methodology described previously (PMID:30833303). A total of 100,240 probes were designed to cover M. smegmatis mc2155 (Agilent probe library ELID number S3206492).
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2021-02-24
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