Analysis of signaling patterns in samples from clinically responding and non-responding patients in the GA study administering single agent tremelimumab.

Phosphoflow results from patients before (Pre) and after (Post) single agent tremelimumab separated among patients with a clinical response (R) or no response (NR). Samples were analyzed by phosphoflow as described in Figure 2. a and b) pZAP70 (pY292) in CD4+ and CD8++ cell subsets. c, d and e) pAkt (pT308) in CD4++, CD8+ and CD14+ cell subsets. f, g, and h) pSTAT6 (Y641) in all three cell subsets. Statistically significant results are denoted with asterisks as described in Figure 2.