Analysis of changes in the BLA and vmPFC brain region of control mice and global cerebral ischemia mice after acute stress. Analysis of changes in the BLA and vmPFC brain region of control mice and global cerebral ischemia mice after acute stress

It is known that the eCB 2-AG is synthesized in postsynaptic cells and released into synaptic clefts when needed. As we observed that acute stress prominently increased eCBs in vmPFC-BLA synapses and that inhibited expression of 2-AG synthase gene Dagla in vmPFC-BLA synapses blocked stress-induced PGCID, we plausibly hypothesized that transient GCI increased 2-AG biosynthesis in BLA neurons, leading to stress-induced increase of 2-AG release into vmPFC-BLA synapses. To test this hypothesis, we employed single-cell sequencing to examine whether GCI mice, in comparison with sham mice, showed increased mRNA expression of the Dagla in c-fos positive BLA and vmPFC cells of acute stress-treated mice Overall design: Seven days after global cerebral ischemia, forced swimming stress was performed, and then BLA and vmPFC were taken for single-cell sequencing analysis