Expression data from HK2 proximal tubule cells transduced with shPGC-1alpha compared to cells transduced with control shRNA

To identify regulation of genes involved in lipid and glycogen metabolism by PGC-1alpha We used whole genome expression analysis to explore the mechanism of lipid and glycogen accumulation in proximal tubule cells following suppression of PGC-1alpha expression. HK2 proximal tubule cell line were transduced with control (shGFP) or shPGC-1alpha shRNA. Successfully infected cells were selected with puromycin. Total cellular RNA was harvested 5 days after transduction, and 24 hours after puromycin selection had been ceased.