Cross-disease integration of single-cell RNA sequencing data from lung myeloid cells reveals TAM signature in in vitro model

Single cells from a 3D human cell-based model comprising tumor cell line-derived spheroids, cancer-associated fibroblasts and primary monocytes were dissociated and analyzed using scRNAseq. 3 monocyte donors were used in the 3D model, and 2 monocyte donors were used for 2D differentiation of macrophages.