Scaling between cell cycle duration and wing growth is regulated by Fat-Dachsous signaling in Drosophila

The atypical cadherins Fat and Dachsous (Ds) signal through the Hippo pathway to regulate growth of numerous organs, including the Drosophila wing. Here, we find that Ds-Fat signaling tunes a unique feature of cell proliferation found to control the rate of wing growth. The duration of the cell cycle increases in direct proportion to the size of the wing, leading to linear rather than exponential growth. Ds-Fat signaling enhances the rate at which the cell cycle lengthens with wing size, thus diminishing the linear rate of wing growth. We show that this results in a complex but stereotyped relative scaling of wing growth with body growth in Drosophila. Finally, we examine the dynamics of Fat and Ds protein distribution in the wing, observing graded distributions that change during growth. However, the significance of these dynamics is unclear since perturbations in expression have negligible impact on wing growth., Data accompanying a published eLife paper titled \"Scaling between cell cycle duration and wing growth is regulated by Fat-Dachsous signaling in Drosophila.\"
 
Imaging data were acquired on confocal micrscope and processed. Please refer to the Methods section of the accompanying eLife paper for detailed methodology of how images were collected. How the data was processed are descripted below. Code is available at https://github.com/andrewliu321/LarvaSeg and https://github.com/andrewliu321/ProteinIntensity.
 
Image processing
 
Raw images were processed using a custom-built Matlab pipeline with no prior preprocessing. The pipeline consists of several modules: 1) surface detection, 2) wing disc, pouch, and midline segmentation, 3) volume measurement, 4) fluorescence intensity measurement, 5) cell segmentation, 6) mitotic index measurement.
 
Surface detection
 
Fat-GFP and Ds-GFP proteins localize to the apical region of cells in the wing disc proper. These proteins are also localized in c..., , # Scaling between cell cycle duration and wing growth is regulated by Fat-Dachsous signaling in Drosophila

[https://elifesciences.org/reviewed-preprints/91572](https://elifesciences.org/reviewed-preprints/91572)

## Summary of the data and file structure

Excel files with fluorescent intensity measured along midlines of wing discs. File name represent genotypes while individual tabs within each excel file specify whether the DV or AP midline was measured. Pay attention to the title row for further specification of age or genotype. 

Excel file titled Animal Properties include various measurements of larvae sizes as well as wing disc measurements, including area, mitotic cells, and cell cycle times.

## Detailed description of each dataset

**Actin_trol_RNAi.xlsx -** all data supplementing the accompanied paper Fig. 3A-E. WPP staged animals ubiquitously express RNAi against *trol* under the control of Actin5c promoter using the Gal4/UAS system.

The \"Dimensions\" sheet:

* *Genotype*: ...