Data and codes for Density-dependent expression of epitranscriptomic, stress, and appetite regulating genes in Atlantic salmon.

Data and codes for Density-dependent expression of epitranscriptomic, stress, and appetite regulating genes in Atlantic salmon.Morgane Frapin1, Laura Quispe1, Joana Troka1, Jenni M. Prokkola1,2, Ossi Laurikainen3,4, Pekka Hyvärinen5, Craig R. Primmer1,6, Tutku Aykanat1, Ehsan Pashay Ahi1Organismal and Evolutionary Biology Research Program, Faculty of Biological and Environmental Sciences, PO Box 56, FI-00014 University of Helsinki, FinlandNatural Resources Institute Finland (Luke), Oulu, FinlandNatural Resources Institute Finland (Luke), Viikki, Helsinki, FinlandDepartment of Environmental and Biological Sciences, Faculty of Science, Forestry and Technology, University of Eastern FinlandNatural Resources Institute Finland (Luke), Paltamo, FinlandInstitute of Biotechnology, Helsinki Institute of Life Sciences (HiLIFE), University of Helsinki, Finland26 Nov 2025densityGenExp.R: R code for analysis.Objects required to run the code:m6A_results: A data frame that contains phenotypic and global m6A percetages of individuals.qPCR_HTpalt_Cq: A data frame that contains phenotypic and gene expression information (Cq values from qPCR output) of individuals.The three sheets contain the raw data ("Cq"), the calculation of the log2RQ ("RQcalculation") and the "log2RQ" values only, respectively. (The last one, "log2RQ", is used in the script)For the sheet "RQcalculation"Col A:H correpond to fish information,Col I:J correspond to the Cq of the housekeeping genes,Col K: correspond to the geometric mean of the housekeeping genes,Col M:AN correspond to the Cq of the target genes,Col AP:BQ correspond to the dCq,Col BS:CT correspond to the ddCq,Col CV:DW correspond to the RQ,Col DY:EZ correspond to the log2RQ