Investigation of reversible histone acetylation and dynamics in gene expression regulation using 3D liver spheroid model

To investigate epigenetic dynamics in a 3D liver spheroid model, liver spheroids were treated with sodium butyrate for 3 days and allowed to recover for 1 week. We then performed gene expression profiling analysis using data obtained from RNA-seq of spheroids at 4 time points Comparative gene expression profiling analysis of RNA-seq data for HepG2 liver spheroids